Angiotensin II Induces C-Reactive Protein Expression via AT1-ROS-MAPK-NF-κB Signal Pathway in Hepatocytes

Angiotensin II Induces C-Reactive Protein Expression via AT1-ROS-MAPK-NF-κB Signal Pathway in Hepatocytes

Blog Article

Background: C-reactive protein (CRP) participates in development of inflammatory diseases.Hepatocytes are a major contributor of circulating CRP.Although angiotensin II (Ang II) is known to evoke inflammatory response, it remains bostik roll-cote unknown whether Ang II induces CRP expression in hepatocytes.

The present study observed effect of Ang II on CRP expression and the related signal pathway in hepatocytes.Methods: mRNA and protein expressions in human hepatocytes were determined with RT-PCR and Western blot respectively.Reactive oxygen species (ROS) was measured using a fluorescence probe.

CRP in liver and serum of rats was determined by immunohistochemistry and ELISA respectively.Results: Ang II induced mRNA and protein expression of CRP apunisw2 in hepatocytes and increased CRP production in liver and CRP level in serum.Losartan reduced Ang II- induced CRP expression in hepatocytes.

Losartan and thenoyltrifluoroacetone decreased Ang II-stimulated ROS production.N-acetylcysteine antagonized Ang II-induced CRP expression.Losartan and N-acetylcysteine inhibited Ang II-activated ERK1/2.

Unlike ERK1/2, only losartan inhibited Ang II-activated JNK.Furthermore, pyrrolidine dithiocarbamate abolished Ang II-induced CRP expression.Conclusion: Ang II has ability to induce CRP expression in hepatocytes in vitro and in vivo through AT1 receptor followed by ROS, MAPK and NF-κB signal pathway.